Genome Sequence of a Nephritogenic and Highly Transformable M49 Strain of Streptococcus pyogenes

@article{McShan2008GenomeSO,
  title={Genome Sequence of a Nephritogenic and Highly Transformable M49 Strain of Streptococcus pyogenes},
  author={William M. McShan and Joseph J. Ferretti and Tadahiro Karasawa and Alexander N. Suvorov and Shao Ping Lin and Biafang Qin and Honggui Jia and Steve Kenton and Fares Najar and Hongming Wu and Julie Christine Scott and B. A. Roe and Dragutin J. Savic},
  journal={Journal of Bacteriology},
  year={2008},
  volume={190},
  pages={7773 - 7785}
}
ABSTRACT The 1,815,783-bp genome of a serotype M49 strain of Streptococcus pyogenes (group A streptococcus [GAS]), strain NZ131, has been determined. This GAS strain (FCT type 3; emm pattern E), originally isolated from a case of acute post-streptococcal glomerulonephritis, is unusually competent for electrotransformation and has been used extensively as a model organism for both basic genetic and pathogenesis investigations. As with the previously sequenced S. pyogenes genomes, three unique… 
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Highly Influential Citations
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Background Citations
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Methods Citations
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132 Citations

Targeted Curing of All Lysogenic Bacteriophage from Streptococcus pyogenes Using a Novel Counter-selection Technique
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A novel counter-selection approach is applied to identify rare S. pyogenes mutants spontaneously cured of select prophage, to allow for the direct examination of bacteriophage relationships within S.pyogenes and further elucidate how the presence of prophages may affect overall streptococcal survival, pathogenicity, and evolution.
Bacteriophage content of M49 strains of Streptococcus pyogenes.
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It is suggested that this specific phage-related virulence gene constellation might be an important factor increasing M49 GAS pathogenicity.
Dissemination of streptococcal pyrogenic exotoxin G (spegg) with an IS-like element in fish isolates of Streptococcus dysgalactiae.
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PC amplification revealed that all strains isolated from moribund fish harbored the streptolysin S structural gene (sagA), and a five-nucleotide deletion mutation was detected in the ORF of the spegg locus of one GCSE strain at the supposed site of IS981SC insertion, resulting in a frameshift mutation.
The Bacteriophages of Streptococcus pyogenes.
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A large and diverse population of endogenous prophages that are vectors for toxins and other virulence factors and occupy multiple attachment sites in the bacterial genomes are uncovered, and the phage-like chromosomal islands (SpyCI) have evolved to mediate a growth-dependent mutator phenotype while acting as global transcriptional regulators.
Insights into Streptococcus pyogenes pathogenesis from transcriptome studies.
TLDR
Differential transcriptional analysis performed on the bacterial side as well as on the host cell side is focused on, providing new insight into the following five topics: gene-expression patterns under infection-relevant conditions, gene- expression patterns in mutant strains compared with wild-type strains, emergence of exceptionally fit GAS clones, and mechanisms underlying shifts from a pharyngeal to invasive GAS lifestyle.
CodY-mediated regulation of Streptococcus pyogenes exoproteins
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The results indicate that CodY alters the abundance of a select group of S. pyogenes exoproteins, including DNases, a protease, and hylauronidase, which together may alleviate starvation by promoting dissemination of the pathogen to nutrient rich environments and by hydrolysis of host macromolecules.
Essential Genes in the Core Genome of the Human Pathogen Streptococcus pyogenes
TLDR
A mariner-based system (Krmit) for en masse monitoring of complex mutant pools by transposon sequencing (Tn-seq) was developed, and a large proportion of GAS essential genes corresponded to key cellular processes and metabolic pathways, and 177 were found conserved within the GAS core genome established from 20 available GAS genomes.
Colonization of the Murine Oropharynx by Streptococcus pyogenes Is Governed by the Rgg2/3 Quorum Sensing System
TLDR
This work identifies a new transcriptional regulatory system governing the ability of S. pyogenes to colonize the nasopharynx and provides knowledge that could help lead to decolonization therapeutics.
CRISPR Inhibition of Prophage Acquisition in Streptococcus pyogenes
TLDR
Limits in CRISPR content could explain the characteristic acquisition of prophages and might contribute to strain-specific pathogenesis in S. pyogenes.
Comparative and Functional Genomic Analysis of Streptococcus Equi and Streptococcus Zooepidemicus : Identifying Novel Vaccine Targets
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This analysis provides evidence of functional loss, changes in the organisation and sequence of genes, and pathogenic specialisation through the acquisition of prophage encoding a phospholipase A2 toxin, and 4 superantigens, and an integrative conjugative element carrying a novel siderophore-like nonribosomal peptide synthetase (NRPS) system.
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