Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

  title={Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation},
  author={Luke A Gilbert and Max A Horlbeck and Britt Adamson and Jacqueline E. Villalta and Yuwen Chen and Evan H. Whitehead and Carla Patricia Guimaraes and Barbara Panning and Hidde L. Ploegh and Michael C. Bassik and Lei S. Qi and Martin Kampmann and Jonathan S. Weissman},
While the catalog of mammalian transcripts and their expression levels in different cell types and disease states is rapidly expanding, our understanding of transcript function lags behind. We present a robust technology enabling systematic investigation of the cellular consequences of repressing or inducing individual transcripts. We identify rules for specific targeting of transcriptional repressors (CRISPRi), typically achieving 90%-99% knockdown with minimal off-target effects, and… CONTINUE READING
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A versatile protein tagging system for signal amplification in single molecule imaging and gene regulation

  • M. E. Tanenbaum, L. A. Gilbert, L. S. Qi, J. S. Weissman, R. D. Vale
  • Cell 159, this issue, 635–646.
  • 2014
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