Genome Editing in Mouse and Rat by Electroporation.

@article{Kaneko2017GenomeEI,
  title={Genome Editing in Mouse and Rat by Electroporation.},
  author={Takehito Kaneko},
  journal={Methods in molecular biology},
  year={2017},
  volume={1630},
  pages={
          81-89
        }
}
Many knock-out/knock-in mouse and rat strains have been produced by genome editing techniques using engineered endonucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Microinjection of engineered endonucleases into pronuclear-stage embryos is required to produce genome-edited rodents and the development of easy, rapid, and high-efficiency methods that do not require… CONTINUE READING