Genome Editing in Mouse and Rat by Electroporation.

  title={Genome Editing in Mouse and Rat by Electroporation.},
  author={Takehito Kaneko},
  journal={Methods in molecular biology},
Many knock-out/knock-in mouse and rat strains have been produced by genome editing techniques using engineered endonucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Microinjection of engineered endonucleases into pronuclear-stage embryos is required to produce genome-edited rodents and the development of easy, rapid, and high-efficiency methods that do not require… CONTINUE READING