Genetics of a novel starch utilisation pathway present in Klebsiella oxytoca.

@article{Fiedler1996GeneticsOA,
  title={Genetics of a novel starch utilisation pathway present in Klebsiella oxytoca.},
  author={Gabriele Fiedler and Markus Pajatsch and August B{\"o}ck},
  journal={Journal of molecular biology},
  year={1996},
  volume={256 2},
  pages={
          279-91
        }
}
A 14.3 kb DNA fragment from Klebsiella oxytoca M5a1 has been cloned and shown to provide Escherichia coli with the capacity for growth on alpha- and beta-cyclodextrins. This fragment is located immediately upstream of the previously identified cgt gene coding for cyclodextrin glycosyltransferase. It contains ten genes (cym) organised in two divergently oriented clusters separated by a non-coding region of 419 bp. Four of the genes code for products homologous to the maltose and linear… 
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Cloning of the cyclodextrin glucanotransferase gene from alkalophilic Bacillus sp. A2-5a and analysis of the raw starch-binding domain
Abstract The cyclodextrin glucanotransferase (CGTase) gene of alkalophilic Bacillus sp. A2-5a was cloned and expressed in Bacillus subtilis ANA-1 as a host. The DNA region included an open reading
Biochemical study of CymJ, the putative regulator of cyclodextrin metabolism in Klebsiella oxytoca M5a1
TLDR
An intriguing finding was that the presence of CymJ in large amount in the cell caused severe cell division inhibition leading to retardation of growth.
Biochemical study of CymJ , the putative regulator of cyclodextrin metabolism in Klebsiella oxytoca M 5 a 1 Tri
TLDR
An intriguing finding was that the presence of CymJ in large amount in the cell caused severe cell division inhibition leading to retardation of growth.
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It was demonstrated by immunoblotting that CymH is located in the cytoplasm and that no signal peptide was cleaved off, and they support the contention of the existence of a specific transport system.
Cloning and transcriptional analysis of the Thermoanaerobacter ethanolicus strain 39E maltose ABC transport system
TLDR
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Novel glucoamylase-type enzymes from Thermoactinomyces vulgaris and Methanococcus jannaschii whose genes are found in the flanking region of the α-amylase genes
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TLDR
A gene encoding a cyclomaltodextrinase (neopullulanase) was cloned from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius ATCC27009 and its nucleotide sequence was determined and it is proposed that CdaA functions as a cytoplasmic cyclomALTodextrinease (EC 3.2.1.54).
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The amino-acid sequences deduced from ctsUV are similar in to a sequence permease and a siigarbi"ding protein for the sugar transport system from 7hermococcus sp.
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