Genetically stable temperature-sensitive mutants of Salmonella typhi induce protection in mice.

@article{Hooke1993GeneticallyST,
  title={Genetically stable temperature-sensitive mutants of Salmonella typhi induce protection in mice.},
  author={Anne Morris Hooke and Mar{\'i}a Cristina Cerquetti and Barbara J. Zeligs and Zhongde Wang and K A Hoberg and Joseph A. Bellanti},
  journal={Vaccine},
  year={1993},
  volume={11 14},
  pages={
          1386-9
        }
}
Temperature-sensitive (ts) mutants of Salmonella typhi were isolated following treatment with nitrosoguanidine, and characterized with respect to cut-off temperature, ts phenotype and reversion frequency. Linkage of the ts mutations to selectable chromosomal markers was established by generalized transduction with bacteriophage phage Vi I, and the appropriate antibiotic resistances were transduced into the ts mutants. Multiple mutant S. typhi were then constructed by combining three independent… CONTINUE READING