Genetically modified Streptococcus mutans for the prevention of dental caries

@article{Hillman2004GeneticallyMS,
  title={Genetically modified Streptococcus mutans for the prevention of dental caries},
  author={Jeffrey D. Hillman},
  journal={Antonie van Leeuwenhoek},
  year={2004},
  volume={82},
  pages={361-366}
}
  • J. Hillman
  • Published 2004
  • Biology, Medicine
  • Antonie van Leeuwenhoek
There are many examples of positive and negative interactions between different species of bacteria inhabiting the same ecosystem. This observation provides the basis for a novel approach to preventing microbial diseases called replacement therapy. In this approach, a harmless effector strain is permanently implanted in the host's microflora. Once established, the presence of the effector strain prevents the colonization or outgrowth of a particular pathogen. In the case of dental caries… 
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96 Citations
Highly Influential Citations
3
Background Citations
51
Methods Citations
8
Results Citations
1

96 Citations

Draft Genome Sequence of Oral Bacterium Streptococcus mutans JH 1140
TLDR
Assembly of the S. mutans JH1140 genome has helped identify novel bacteriocins and provided further understanding of the effector strain BCS3-L1’s superior colonization properties that facilitated its development as a replacement therapy strain.
Virulence properties of Streptococcus mutans.
  • J. Banas
  • Biology
    Frontiers in bioscience : a journal and virtual library
  • 2004
TLDR
This review focuses on the bacterial components that contribute to each of the major virulence properties of S. mutans and describes how these components work together in the development of dental caries.
A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries
TLDR
Findings demonstrate that MS-gcrR-def appears to be a good candidate for replacement therapy, and shows that the adhesion ability of the mutant increased relative to the wild type, especially in the early stage.
The mutacins of Streptococcus mutans: regulation and ecology.
TLDR
As more S. mutans strains are sequenced, it is anticipated that additional mutacins with novel functions will be discovered, which may yield further insights into the ecological role ofmutacins within the oral biofilm.
Identification of genes associated with mutacin I production in Streptococcus mutans using random insertional mutagenesis.
TLDR
The results suggest that mutacin I production is stringently controlled by diverse and complex regulatory pathways.
Genotyping and Phenotypic Analysis of Streptococcus mutans Isolates From Children With and Without Dental Caries
TLDR
Within dental plaque, Streptococcus mutans produces acids from fermentable carbohydrates, decreasing the biofilm pH, causing enamel demineralization, and the ability of S. mutans to produce high levels of persisters may be a hallmark of cariogenicity.
Modification of an effector strain for replacement therapy of dental caries to enable clinical safety trials
TLDR
The strain has significantly reduced cariogenicity owing to a deletion of the entire open reading frame for lactate dehydrogenase, and has excellent colonization potential through the production of a natural antibiotic called mutacin 1140.
Screening for inhibitors of mutacin synthesis in Streptococcus mutans using fluorescent reporter strains
TLDR
The successful development of a whole-cell fluorescent reporter for the screening of natural compounds and report that erinacine C suppresses mutacin synthesis in S. mutans without affecting cell viability are demonstrated.
Salivaricin E and abundant dextranase activity may contribute to the anti-cariogenic potential of the probiotic candidate Streptococcus salivarius JH.
TLDR
Strain JH produces salivaricin E, a 32 aa lantibiotic with a mass of 3565.9 Da, which is responsible for the inhibition of S. mutans growth, and dextranase, an enzyme that hydrolyses (1 → 6)-α-D-glucosidic linkages, at levels higher than any other S. salivarius tested.
The vaccination approach to control infections leading to dental caries
TLDR
The advances in understanding of the molecular mechanisms which underlie MS transmission, tooth colonization and virulence are discussed, and current strategies for anti-caries vaccination efforts with regard to important bacterial targets, routes, adjuvants and delivery systems for active and passive immunization are described.
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References

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