Genetically engineering transferrin to improve its in vitro ability to deliver cytotoxins.

@article{Yoon2009GeneticallyET,
  title={Genetically engineering transferrin to improve its in vitro ability to deliver cytotoxins.},
  author={Dennis J. Yoon and David S. H. Chu and Christopher W. Ng and Edward A Pham and Anne B Mason and David M Hudson and Valerie C. Smith and R. T. MacGillivray and Daniel T. Kamei},
  journal={Journal of controlled release : official journal of the Controlled Release Society},
  year={2009},
  volume={133 3},
  pages={178-84}
}
We previously demonstrated that decreasing the iron release rate of transferrin (Tf), by replacing the synergistic anion carbonate with oxalate, increases its in vitro drug carrier efficacy in HeLa cells. In the current work, the utility of this strategy has been further explored by generating two Tf mutants, K206E/R632A Tf and K206E/K534A Tf, exhibiting different degrees of iron release inhibition. The intracellular trafficking behavior of these Tf mutants has been assessed by measuring their… CONTINUE READING

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