Genetically engineered immunoglobulins reveal structural features controlling segmental flexibility.

@article{Schneider1988GeneticallyEI,
  title={Genetically engineered immunoglobulins reveal structural features controlling segmental flexibility.},
  author={W P Schneider and Theodore G Wensel and Lubert Stryer and Vernon T. Oi},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1988},
  volume={85 8},
  pages={2509-13}
}
We have carried out nanosecond fluorescence polarization studies of genetically engineered immunoglobulins to determine the structural features controlling their segmental flexibility. The proteins studied were hybrids of a relatively rigid isotype (mouse IgG1) and a relatively flexible one (mouse IgG2a). They have identical light chains and heavy chain variable regions and have the same combining sites for epsilon-dansyl-L-lysine, a fluorescent hapten. The fluorescence of the bound dansyl… CONTINUE READING

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