Genetically engineered avidins and streptavidins

  title={Genetically engineered avidins and streptavidins},
  author={O. Laitinen and V. Hyt{\"o}nen and H. Nordlund and M. Kulomaa},
  journal={Cellular and Molecular Life Sciences CMLS},
  • O. Laitinen, V. Hytönen, +1 author M. Kulomaa
  • Published 2006
  • Biology, Medicine
  • Cellular and Molecular Life Sciences CMLS
  • Abstract.Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification and labeling techniques to diagnostics, and from targeted drug delivery to nanotechnology. (Strept)avidin-biotin technology relies on the extremely tight and specific affinity between (strept)avidin and biotin (dissociation constant, Kd≈10−14–10−16 M). (Strept)avidins are also exceptionally stable proteins. To study their ligand… CONTINUE READING
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