Genetically encoded indicators of cellular calcium dynamics based on troponin C and green fluorescent protein.

@article{Heim2004GeneticallyEI,
  title={Genetically encoded indicators of cellular calcium dynamics based on troponin C and green fluorescent protein.},
  author={Nicola Heim and Oliver Griesbeck},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 14},
  pages={14280-6}
}
Genetic calcium probes offer tremendous potential in the fields of neuroscience, cell biology, and pharmaceutical screening. Previously, ratiometric and non-ratiometric indicators of cellular calcium dynamics have been described that consist of mutants of the green fluorescent protein (GFP) as fluorophores and calmodulin as calcium-binding moiety in several configurations. However, these calmodulin-based types of probes have a series of deficiencies, such as reduced dynamic ranges, when… CONTINUE READING
Highly Influential
This paper has highly influenced a number of papers. REVIEW HIGHLY INFLUENTIAL CITATIONS