Genetically encoded fluorescent sensors for intracellular NADH detection.

@article{Zhao2011GeneticallyEF,
  title={Genetically encoded fluorescent sensors for intracellular NADH detection.},
  author={Yuzheng Zhao and Jing Jin and Qingxun Hu and Hai-Meng Zhou and Jing Yi and Zhenhang Yu and Lei Xu and Xue Wang and Y. S. Yang and Joseph Loscalzo},
  journal={Cell metabolism},
  year={2011},
  volume={14 4},
  pages={555-66}
}
We have developed genetically encoded fluorescent sensors for reduced nicotinamide adenine dinucleotide (NADH), which manifest a large change in fluorescence upon NADH binding. We demonstrate the utility of these sensors in mammalian cells by monitoring the dynamic changes in NADH levels in subcellular organelles as affected by NADH transport, glucose metabolism, electron transport chain function, and redox environment, and we demonstrate the temporal separation of changes in mitochondrial and… CONTINUE READING
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