Genetically encoded bright Ca2+ probe applicable for dynamic Ca2+ imaging of dendritic spines.

Abstract

G-CaMP is a Ca2+ probe based on a single green fluorescent protein (GFP). G-CaMP shows a large fluorescence increase upon Ca2+ binding, but its fluorescence is dim and pH sensitive, similar to other single GFP-based probes. Here we report an improved G-CaMP, named G-CaMP1.6, which enables easier detection of intracellular Ca2+ signals. G-CaMP1.6 was… (More)

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Cite this paper

@article{Ohkura2005GeneticallyEB, title={Genetically encoded bright Ca2+ probe applicable for dynamic Ca2+ imaging of dendritic spines.}, author={Masamichi Ohkura and Masanori Matsuzaki and Haruo Kasai and Keiji Imoto and Junichi Nakai}, journal={Analytical chemistry}, year={2005}, volume={77 18}, pages={5861-9} }