Genetically encoded Ca(2+) indicators: properties and evaluation.

@article{Koldenkova2013GeneticallyEC,
  title={Genetically encoded Ca(2+) indicators: properties and evaluation.},
  author={Vadim P{\'e}rez Koldenkova and Takeharu Nagai},
  journal={Biochimica et biophysica acta},
  year={2013},
  volume={1833 7},
  pages={1787-97}
}
Genetically encoded calcium ion (Ca(2+)) indicators have become very useful and widely used tools for Ca(2+) imaging, not only in cellular models, but also in living organisms. However, the in vivo and in situ characterization of these indicators is tedious and time consuming, and it does not provide information regarding the suitability of an indicator for particular experimental environments. Thus, initial in vitro evaluation of these tools is typically performed to determine their properties… CONTINUE READING