Genetically Targeted All-Optical Electrophysiology with a Transgenic Cre-Dependent Optopatch Mouse.

@article{Lou2016GeneticallyTA,
  title={Genetically Targeted All-Optical Electrophysiology with a Transgenic Cre-Dependent Optopatch Mouse.},
  author={Shan Lou and Yoav Adam and Eli N Weinstein and Erika K. Williams and Katherine Williams and Vicente Parot and Nikita Kavokine and Stephen D. Liberles and Linda Madisen and Hongkui Zeng and Adam E Cohen},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  year={2016},
  volume={36 43},
  pages={11059-11073}
}
Recent advances in optogenetics have enabled simultaneous optical perturbation and optical readout of membrane potential in diverse cell types. Here, we develop and characterize a Cre-dependent transgenic Optopatch2 mouse line that we call Floxopatch. The animals expressed a blue-shifted channelrhodopsin, CheRiff, and a near infrared Archaerhodopsin-derived voltage indicator, QuasAr2, via targeted knock-in at the rosa26 locus. In Optopatch-expressing animals, we tested for overall health… CONTINUE READING
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