• Corpus ID: 53964523

Genetic variability in sugarcane plantlets developed through in vitro mutagenesis.

@article{Khan2009GeneticVI,
  title={Genetic variability in sugarcane plantlets developed through in vitro mutagenesis.},
  author={Imran Khan and Muhammad Umar Dahot and Nighat Seema and Shafqat Yasmin and Sajida Bibi and Saboohi Raza and Abdullah. Khatri},
  journal={Pakistan Journal of Botany},
  year={2009},
  volume={41},
  pages={153-166}
}
Abstarct Three sugarcane clones viz., NIA-98, NIA-2004 and BL4 were used for induction of genetic variability through In-vitro mutagenesis. Apical meristametic region was used for callus induction (4mg/l 2,4-D). Actively growing callus was treated with five different doses of gamma rays (10Gy, 20Gy, 30Gy, 40Gy and 50Gy). Maximum callus proliferation and plantlets regeneration were recorded in 20Gy and minimum at 50Gy. Maximum chlorophyll mutation frequency was noted in 40 and 50 Gy. The… 

Figures and Tables from this paper

PLANT REGENERATION FROM IRRADIATED EMBRYOGENIC CALLUS OF SUGARCANE

Three sugarcane clones were used for induction of genetic variability through In-vitro mutagenesis and apical meristametic region was used for callus induction on 4mg/l 2,4-D.

In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers

A combination of irradiation, regeneration, multiplication, and random amplification of polymorphic DNA (RAPD) analysis for early screening of mutants can speed up the breeding program of E. elatior.

Irradiation induced mutagenesis in sugarcane

It is inferred that genetic variability could be induced in sugarcane using gamma rays for traits like germination, cane height, leaf length, HR brix, single cane weight and to red rot tolerance.

EVALUATION OF SUGARCANE (SACCHARUM SPP. COMPLEX) MUTANTS FOR YIELD, YIELD CONTRIBUTING TRAITS AND QUALITY PARAMETERS

In the present investigation, field grown mutants were characterized for yield and quality contributing traits in comparison to parent variety Co 99004 and Co 94012. The MS + 2, 4-D (4.0 mg/l) +

Induced mutations and somaclonal variations in three sugarcane (Saccharum officinarum L.) varieties.

Hybridizing the genotypes from different clusters of the analysis can be of promising outcomes in terms of getting the genetic diversity among progeny plants for further evaluation in cane breeding programs.

IN VITRO MUTAGENESIS IN BANANA AND VARIANT SCREENING THROUGH ISSR

An efficient culture medium for In vitro clonal propagation of banana cultivars was established. Meristamtic tips of Basrai and GCTCV-215 were irradiated with different doses of gamma rays viz., 0,

Revisiting the physical mutagenesis for sugarcane improvement: a stomatal prospective

It is suggested that gamma radiations cause variations in stomatal characteristics of sugarcane that further influence the photosynthetic activity and instigate a direct impact on the agronomic performance of the crop.

Field assessment of agronomic traits and in vitro acetolactate synthase characterisation of imazapyr herbicide tolerant sugarcane.

This study identified which of the seven herbicide mutant lines of variety N12 had agronomic characteristics at least equivalent to un-mutated N12, and determined the effect of residual soil herbicide activity on germination of sugarcane setts.

INFLUENCE OF PHYTOHORMONE ON THE ORGANOGENESIS OF SUGARCANE

Three sugarcane varieties were used in this study in order to compare their response to different concentration of phytohormones (Dicamba 2-4D and Picloram), and best callus induction was observed using dicamba fallowed by 2,4-D whereas best proliferation was achieved with dicnda.

In vitro regeneration, detection of somaclonal variation and screening for mosaic virus in sugarcane ( Saccharum spp.) somaclones

It is demonstrated that SSR genetic markers are the best tool for the investigation of genetic variation in sugarcane.
...

References

SHOWING 1-10 OF 45 REFERENCES

Plant regeneration via somatic embryogenesis in sugarcane

Calli grown on MSC3 medium, when transferred to half-MS medium containing 15 g 1−1 sucrose, produced tiny plantlets without forming coleoptiles, suggesting precocious germination of somatic embryos, and the regenerates included morphological variants.

Agronomic performance and molecular assessment of tissue culture‐derived barley lines

The results indicate that the agronomic performance was altered by tissue culture regeneration and the frequency of variation was cultivar dependent.

In-vitro Techniques and Mutagenesis for the Improvement of Vegetatively Propagated Plants

A number of important crops such as banana, plantain, date palm, citrus, potato, sweet potato, pineapple, apple, and pear are propagated from vegetative parts, and are not amenable to improvement in the same manner as sexually propagated plants, so mutation induction offers the possibility to alter a few characters without disrupting the genome.

Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets

Analysis of random-amplified polymorphic DNA (RAPD) using 21 arbitrary oligonucleotide 10-mers showed the genetic homogeneity of P. notoginseng, suggesting that somatic embryogenesis can be used for clonal micropropagation of this plant.

PERFORMANCE OF PROMISING SUGARCANE CLONE FOR YIELD AND QUALITY CHARACTERS 11 . STABILITY STUDIES

A new sugarcane clone AEC81-8415, generated from seed (fuzz) of a croos combination of NCo 310 x CP56-614, imported from ARS, USDA, Canal Point, Florida, USA, alongwith other genotypes was evaluated

Une methode de planification experimentale appliquee aux cultures de tissus vegetaux. Exemple de la canne a sucre (Saccharum sp.)

Fragments of roots and shoot tips were taken from sugarcane plants propagated in vitro and the effects of light, 4-dichlorophenoxyacetic acid and 6-benzyl-aminopurine on initiation and growth of calli were observed.

Chromosomal variability in tissue cultures and regenerated plants of Hordeum

  • T. Orton
  • Biology, Medicine
    Theoretical and Applied Genetics
  • 2004
In vitro chromosomal variability was observed in callus and suspension cultures of Hordeum vulgare, H. jubatum, and their interspecific hybrid, and the extent to which each class of chromosome variability was present in a culture depended upon differentiated state, age, and history.

Genetic and molecular analysis of tissue-culture-derived Ac elements

The objective of the present study was to determine whether the presence of Ac activity in tissue-culture-derived plants was associated with changes in the number or structure of Ac-homologous DNA sequences.

Molecular markers applied to plant tissue culture

This review describes the use of molecular markers to address fundamental and practical questions of plant tissue culture, and discusses the potential of improvements in molecular techniques and new molecular markers such as SCAR and STS along with high-resolution mapping strategies.