Genetic variability and evolution of the Schistosoma genome analysed by using random amplified polymorphic DNA markers.

@article{Barral1993GeneticVA,
  title={Genetic variability and evolution of the Schistosoma genome analysed by using random amplified polymorphic DNA markers.},
  author={V J Barral and Patrice This and Dani{\`e}le Imbert-Establet and Claude Combes and Michel Delseny},
  journal={Molecular and biochemical parasitology},
  year={1993},
  volume={59 2},
  pages={211-21}
}
The usefulness of random amplified polymorphic DNA markers (RAPD) was assayed in an attempt to discriminate among species, strains and individuals within the genus Schistosoma. Depending on the species, 40-50 arbitrary decamer oligonucleotides were used as primers to amplify total DNA by the polymerase chain reaction (PCR). An important polymorphism was observed among 5 species, allowing a phylogenetic tree to be outlined. These differences can be used for rapid and accurate identification. A… CONTINUE READING

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