Genetic typing of the mouse ob mutation by PCR and restriction enzyme analysis.

@article{Hirasawa1997GeneticTO,
  title={Genetic typing of the mouse ob mutation by PCR and restriction enzyme analysis.},
  author={Tsutomu Hirasawa and Tatsuji Ohara and Susumu Makino},
  journal={Experimental animals},
  year={1997},
  volume={46 1},
  pages={75-8}
}
A genetic typing method for the mouse obese (ob) mutation by PCR and restriction fragment length polymorphism (RFLP) analysis was developed. Three genotypes (ob/ob, ob/+ and +/+) of the ob mouse are rapidly differentiated with this assay. Since only a small biopsy specimen (tail end) is necessary for the genotyping, the ob mouse at any age is typable and can be used in subsequent breeding or research. Obese homozygotes (ob/ob) were efficiently produced by mating heterozygotes (ob/+) only, which… CONTINUE READING

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