Genetic transformation using maize immature zygotic embryos.

@article{Frame2011GeneticTU,
  title={Genetic transformation using maize immature zygotic embryos.},
  author={B. Frame and Marcy Main and R. Schick and Kan Wang},
  journal={Methods in molecular biology},
  year={2011},
  volume={710},
  pages={
          327-41
        }
}
Epidermal and subepidermal cells in the abaxial, basal region of the maize (Zea mays L.) immature zygotic embryo (IZE) scutellum can be induced by exogenous auxin to proliferate and undergo somatic embryogenesis. Successful genetic transformation of IZEs depends not only on optimizing transformation parameters for these totipotent cells, but also on achieving high embryogenic callus induction frequency (ECIF) in a population of targeted explants. In maize, ECIF is strongly influenced by… Expand
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References

SHOWING 1-10 OF 47 REFERENCES
Agrobacterium-mediated transformation of maize
...
1
2
3
4
5
...