Genetic transformation of mouse cultured cells with the help of high-velocity mechanical DNA injection.

@article{Zelenin1989GeneticTO,
  title={Genetic transformation of mouse cultured cells with the help of high-velocity mechanical DNA injection.},
  author={Alexander V. Zelenin and A V Titomirov and Vladimir A. Kolesnikov},
  journal={FEBS letters},
  year={1989},
  volume={244 1},
  pages={65-7}
}
NIH 3T3 mouse cells were transfected by the plasmid pSV3neo (G418-resistant) with the help of high-velocity mechanical DNA injection based on the principle of bombarding cells with tungsten particles covered with the DNA. Stable transformants were obtained. Dot-hybridization and Southern analysis revealed the integration into the genome of 5-20 copies per cell of original plasmid DNA. The plasmid DNA was shown to have tandem organization. 

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