Genetic transformation of Norway spruce (Picea abies (L.) Karst) using somatic embryo explants by microprojectile bombardment

@article{Robertson1992GeneticTO,
  title={Genetic transformation of Norway spruce (Picea abies (L.) Karst) using somatic embryo explants by microprojectile bombardment},
  author={Dominique Robertson and A. Keyton Weissinger and Rhonda Ackley and Sarah C Glover and Ronald R. Sederoff},
  journal={Plant Molecular Biology},
  year={1992},
  volume={19},
  pages={925-935}
}
Stable transformation of Norway spruce tissue has been obtained following bombardment of mature somatic embryos with pRT99gus, a plasmid that contains neo coding for NPTII, and gusA, coding for β-glucuronidase, both fused to the CaMV 35S promoter. At least 8 lines have been stably transformed (over 15 months in culture) following bombardment and selection on kanamycin. Polymerase chain reaction analyses showed a high frequency of cotransformation of the gusA and neo genes. The frequency of… CONTINUE READING

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