Genetic transformation of Cymbidium orchid by particle bombardment

  title={Genetic transformation of Cymbidium orchid by particle bombardment},
  author={Jinfen Yang and H.-J. Lee and Dong Hyun Shin and Soo Kyung Oh and Jeong Hoon Seon and Kee Yoeup Paek and K. Han},
  journal={Plant Cell Reports},
 A protocol is presented for genetically engineering Cymbidium orchid using particle bombardment. This protocol enabled the routine transformation of orchid plants that were previously difficult to transform. Liquid culture was used to generate a large number of protocorm-like bodies (PLBs) to be bombarded and to promote continued development of the bombarded meristematic tissue. Plasmid DNA (pKH200) carrying the GUS-INT and NPTII genes flanked by tobacco matrix attachment regions was… CONTINUE READING