Genetic transformation in the grain legume Cicer arietinum L. (chickpea)

  title={Genetic transformation in the grain legume Cicer arietinum L. (chickpea)},
  author={Giovanna S. Fontana and Luca Santini and Sofia Caretto and Giovanna Frugis and Domenico Mariotti},
  journal={Plant Cell Reports},
In the grain legume Cicer arietinum L. (chickpea), the seed-derived embryo axes deprived of the apical meristem were able to regenerate adventitious shoots on Murashige and Skoog (1962) medium supplemented with kinetin. This protocol was suitable for Agrobacterium-mediated gene transfer by the co-cultivation technique. Chickpea transgenic plants showed neomycin phosphotransferase II and ß-glucuronidase activities and the presence in their genome of integrated bacterial DNA. 
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