Genetic transformation and gene silencing mediated by multiple copies of a transgene in eastern white pine.

@article{Tang2007GeneticTA,
  title={Genetic transformation and gene silencing mediated by multiple copies of a transgene in eastern white pine.},
  author={Wei Tang and Ronald J. Newton and Douglas A. Weidner},
  journal={Journal of experimental botany},
  year={2007},
  volume={58 3},
  pages={545-54}
}
An efficient transgenic eastern white pine (Pinus strobus L.) plant regeneration system has been established using Agrobacterium tumefaciens strain GV3850-mediated transformation and the green fluorescent protein (gfp) gene as a reporter in this investigation. Stable integration of transgenes in the plant genome of pine was confirmed by polymerase chain reaction (PCR), Southern blot, and northern blot analyses. Transgene expression was analysed in pine T-DNA transformants carrying different… CONTINUE READING

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transgene silencing

  • V Levee, E Garin, K Klimaszewska, A. Seguin
  • The Plant Journal
  • 1999
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