Genetic tools for Sulfolobus spp.: vectors and first applications

  title={Genetic tools for Sulfolobus spp.: vectors and first applications},
  author={Silvia Berkner and Georg Lipps},
  journal={Archives of Microbiology},
Sulfolobus species belong to the best-studied archaeal organisms but have lacked powerful genetic methods. Recently, there has been considerable progress in the field of Sulfolobus genetics. Urgently needed basic genetic tools, such as targeted gene knockout techniques and shuttle vectors are being developed at an increasing pace. For S. solfataricus knockout systems as well as different shuttle vectors are available. For the genetically more stable S. acidocaldarius shuttle vectors have been… CONTINUE READING
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