Genetic surface-display of methyl parathion hydrolase on Yarrowia lipolytica for removal of methyl parathion in water

@article{Wang2012GeneticSO,
  title={Genetic surface-display of methyl parathion hydrolase on Yarrowia lipolytica for removal of methyl parathion in water},
  author={Xing-xing Wang and Zhe Chi and S H Ru and Zhenming Chi},
  journal={Biodegradation},
  year={2012},
  volume={23},
  pages={763-774}
}
In this study, the mph gene encoding methyl parathion hydrolase from Pseudomonas sp. WBC-3 was expressed in Yarrowia lipolytica and the expressed methyl parathion hydrolase was displayed on cell surface of Y. lipolytica. The activity of methyl parathion hydrolase displayed on the yeast cells of the transformant Z51 was 59.5 U mg−1 of cell dry cells (450.6 U per mL of the culture) in the presence of 5.0 mM of Co2+. The displayed methyl parathion hydrolase had the optimal pH of 9.5 and the… CONTINUE READING

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