Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells

@article{Loi2001GeneticRO,
  title={Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells},
  author={P. Loi and G. Ptak and B. Barboni and J. Fulka and P. Cappai and M. Clinton},
  journal={Nature Biotechnology},
  year={2001},
  volume={19},
  pages={962-964}
}
Since the advent of procedures for cloning animals, conservation biologists have proposed using this technology to preserve endangered mammals. Here we report the successful cloning of a wild endangered animal, Ovis orientalis musimon, using oocytes collected from a closely related, domesticated species, Ovis aries. We injected enucleated sheep oocytes with granulosa cells collected from two female mouflons found dead in the pasture. Blastocyst-stage cloned embryos transferred into sheep foster… Expand
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