Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells

  title={Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells},
  author={P. Loi and G. Ptak and B. Barboni and J. Fulka and P. Cappai and M. Clinton},
  journal={Nature Biotechnology},
  • P. Loi, G. Ptak, +3 authors M. Clinton
  • Published 2001
  • Biology, Medicine
  • Nature Biotechnology
  • Since the advent of procedures for cloning animals, conservation biologists have proposed using this technology to preserve endangered mammals. Here we report the successful cloning of a wild endangered animal, Ovis orientalis musimon, using oocytes collected from a closely related, domesticated species, Ovis aries. We injected enucleated sheep oocytes with granulosa cells collected from two female mouflons found dead in the pasture. Blastocyst-stage cloned embryos transferred into sheep foster… CONTINUE READING
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