Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells

@article{Loi2001GeneticRO,
  title={Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells},
  author={Pasqualino Loi and Grażyna Ewa Ptak and Barbara Barboni and Josef Fulka and Pietro Cappai and Michael Clinton},
  journal={Nature Biotechnology},
  year={2001},
  volume={19},
  pages={962-964}
}
Since the advent of procedures for cloning animals, conservation biologists have proposed using this technology to preserve endangered mammals. Here we report the successful cloning of a wild endangered animal, Ovis orientalis musimon, using oocytes collected from a closely related, domesticated species, Ovis aries. We injected enucleated sheep oocytes with granulosa cells collected from two female mouflons found dead in the pasture. Blastocyst-stage cloned embryos transferred into sheep foster… 
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TLDR
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The rescue of an endangered species, the European mouflon (Ovis orientalis musimon), is described for the first time by the application of an integrated package of reproductive biotechnologies.
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TLDR
There are controversial reports in the literature, but in most cases these experiments have underlined some of the cellular and molecular mechanisms that are incomplete during cell nucleus reprogramming, including the failure to organize nucleoli, silence somatic cell genes, activate the embryonic genome, and resume mitochondrial replication and function, thus indicating nucleus-cytoplasmic incompatibility.
Somatic cell cloning for livestock and endangered species.
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Research of the SCNT in livestock and endangered species, including the current work, will be discussed.
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