Genetic relationships among species of the genus Diplotaxis (Brassicaceae) using inter-simple sequence repeat markers

  title={Genetic relationships among species of the genus Diplotaxis (Brassicaceae) using inter-simple sequence repeat markers},
  author={Juan Pedro Mart{\'i}n and Mar{\'i}a Dolores S{\'a}nchez-Y{\'e}lamo},
  journal={Theoretical and Applied Genetics},
Inter-simple sequence repeat (ISSR) amplification was evaluated for its applicability as a genetic marker system to establish relationships among ten Diplotaxis species. ISSR amplification generated multiple banding profiles with the 12 primers from all DNA samples, with an average of 41.2 fragments per primer. This average was clearly higher for the 5´ triple-anchored primers than for other primers. The banding profiles were highly repeatable across separate PCR runs. DNA mixing procedures… CONTINUE READING


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