Genetic relatedness of Colorado tick fever virus isolates by RNA-RNA blot hybridization.

@article{Bodkin1987GeneticRO,
  title={Genetic relatedness of Colorado tick fever virus isolates by RNA-RNA blot hybridization.},
  author={D K Bodkin and Dennis L. Knudson},
  journal={The Journal of general virology},
  year={1987},
  volume={68 ( Pt 4)},
  pages={1199-204}
}
The sequence relatedness of ten isolates of the Colorado tick fever (CTF) serogroup of orbiviruses was examined by RNA-RNA blot hybridization. The 12 dsRNA genome segments of each of the isolates were electrophoresed in a 10% polyacrylamide gel, the segments were transferred electrophoretically to membranes and hybridized to radiolabelled genomic RNA from CTF Florio mouse-adapted strain (CTF FMA) or CTF SS-18. All genome segments of the ten CTF viruses exhibited cross-hybridization signals with… CONTINUE READING