• Biology, Medicine
  • Published in
    Applied and environmental…
    1992

Genetic relatedness of Bradyrhizobium japonicum field isolates as revealed by repeated sequences and various other characteristics.

@article{Minamisawa1992GeneticRO,
  title={Genetic relatedness of Bradyrhizobium japonicum field isolates as revealed by repeated sequences and various other characteristics.},
  author={Ken Minamisawa and Tatsuji Seki and Shiroh Onodera and Masaru Kubota and Teruo Asami},
  journal={Applied and environmental microbiology},
  year={1992},
  volume={58 9},
  pages={
          2832-9
        }
}
Forty-nine isolates of Bradyrhizobium japonicum indigenous to a field where soybeans were grown for 45 years without inoculation were characterized by using four DNA hybridization probes from B. japonicum. nifDK-specific hybridization clearly divided the isolates into two divergent groups. Diversity in repeated-sequence (RS)-specific hybridization was observed; 44 isolates derived from 41 nodules were divided into 33 different RS fingerprint groups. Cluster analysis showed that the RS… CONTINUE READING

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