Genetic polymorphism and taxonomic infrastructure of the Pleurotus eryngii species-complex as determined by RAPD analysis, isozyme profiles and ecomorphological characters.

@article{Zervakis2001GeneticPA,
  title={Genetic polymorphism and taxonomic infrastructure of the Pleurotus eryngii species-complex as determined by RAPD analysis, isozyme profiles and ecomorphological characters.},
  author={Georgios I. Zervakis and Giuseppe Venturella and Kalliopi K Papadopoulou},
  journal={Microbiology},
  year={2001},
  volume={147 Pt 11},
  pages={
          3183-94
        }
}
The Pleurotus eryngii species-complex includes populations of choice edible mushrooms, growing in the greater Mediterranean area in close association with different genera of plants of the family Apiaceae. Their distinct host-specialization served as the principal criterion for the discrimination of several taxa; however, the genetic relationships among the various P. eryngii ecotypes remain ambiguous. In the present study, 46 Pleurotus strains with a wide range of geographical origins were… 

Figures and Tables from this paper

Genetic variability and molecular phylogeny of Pleurotus eryngii species-complex isolates from Iran, and notes on the systematics of Asiatic populations
TLDR
Pleurotus populations growing on umbellifers in Iran seem either to have recently diverged through a sympatric speciation process based mainly on ecological factors, or they form part of a rather wide agglomerate associated with various host-plants where exchange of genetic material is still in progress.
Species delimitation in Pleurotus eryngii species-complex inferred from ITS and EF-1α gene sequences
TLDR
Data from ITS and EF1-α genes supported the hypothesis that the Pleurotus eryngii species-complex is a unique gene-pool, and the nebrodensis type is characterized by a level of nucleotide substitutions lower than as expected for its ranking at a species level.
Genetic diversity and population structure of Iranian wild Pleurotus eryngii species- complex strains revealed by URP-PCR markers
TLDR
Analysis of molecular variance (AMOVA) revealed significant among regions, among populations within regions and within population diversity, whereas within population variation (61.6%) accounted for most of the total molecular variance.
DNA-fingerprinting (AFLP and RFLP) for genotypic identification in species of the Pleurotus eryngii complex
TLDR
Both laccase RFLP and AFLP have been proved to distinguish unambiguously the three taxa studied and showed enough sensitivity to detect polymorphisms among the strains, proving to be an efficient DNA fingerprinting tool in studies of strain assignment.
Evaluation of Genetic Diversity Among Chinese Pleurotus eryngii Cultivars by Combined RAPD/ISSR Marker
TLDR
Combined randomly amplified polymorphic DNA (RAPD)/inter-simple sequence repeat (ISSR) analysis contributed to a better understanding of the genetic relationships among the P. eryngii strains and provide orientation for the strain improvement of the species.
Molecular classification and phylogenetic relationships of selected edible Basidiomycetes species
TLDR
This study proves that the sequence of the ITS region is a superior molecular DNA barcode for taxonomic identification of Basidiomycetes and can be clearly differentiated within the families as well as genera.
Genetic structure of the Pleurotus eryngii species-complex.
TLDR
Qualitative morphological traits were efficient in differentiating isolates of P. eryngii, var.
...
...

References

SHOWING 1-10 OF 91 REFERENCES
Molecular systematics of the genus Pleurotus: analysis of restriction polymorphisms in ribosomal DNA.
TLDR
These results support the common genetic background of P. pulmonarius, P. sajor-caju and P. sapidus and seem to be the appropriate tool for both varietal characterization and protection of commercially valuable strains.
Isozyme variation and species delimitation in the Ceratocystis coerulescens complex.
Nineteen electrophoretic phenotypes (unique combinations of electromorphs) were found among 98 isolates of Ceratocystis coerulescens and mor? phologically similar species using 10 isozymes. Analysis
Taxonomic relationships within the fungal genus Pleurotus as determined by isoelectric focusing analysis of enzyme patterns
TLDR
Isoenzymes from 23 isolates belonging to the fungal genus Pleurotus, subdivision Basidiomycotina, were examined by polyacrylamide gel isoelectric focusing and protein blotting, and the resulting dendrogram of taxonomic distances demonstrated the suitability of enzyme isoeLECTric focusing for use in systematics.
RAPD marker and isozyme characterization of Sphaeropsis sapinea from diverse coniferous hosts and locations
TLDR
This is the first report of isolates from hosts other than Pinus banksiana or P. resinosa, and from outside Michigan, Minnesota, or Wisconsin, that have been characterized as members of the RAPD marker group B.
Isozyme detection and variation in Leucostoma species from Prunus and Malus
Isozyme analysis was used to study the vari? ation among isolates of Leucostoma causing canker on fruit trees. Seventy-seven isolates including 17 from teleomorphs were obtained from six Prunus
Differentiation of Fusarium oxysporum f. sp. vasinfectum races on cotton by random amplified polymorphic DNA (RAPD) analysis
TLDR
It is suggested that RAPD markers can be a quick and reliable alternative for differentiating isolates of F. o.
Population genetics and systematics of the Morchella esculenta complex.
TLDR
Strains of the two different forms do not cluster separately in a phenetic analysis indicating that the two phenotypes are not different taxa, demonstrating that they exist as Mendelian populations.
Differentiation of isolates of Discula umbrinella (Teleomorph: Apiognomonia errabunda) from beech, chestnut, and oak using randomly amplified polymorphic DNA markers.
TLDR
Cluster analysis of all arbitrary primed amplified DNA fragments showed that the isolates could be placed into four groups corresponding to their host origin, and the high percentage of private RAPD variants within groups is consistent with low gene flow.
...
...