Genetic polymorphism across regions of the three open reading frames of “Norwalk-like viruses”

  title={Genetic polymorphism across regions of the three open reading frames of “Norwalk-like viruses”},
  author={Jan Vinj{\'e} and J. Green and D. Lewis and Chris I. Gallimore and D. W. G. Brown and Marion P. G. Koopmans},
  journal={Archives of Virology},
Summary. Genomic characterization of Norwalk-like human caliciviruses (NLVs) originating from outbreaks and sporadic cases of acute gastroenteritis has revealed surprisingly high levels of diversity, even in the RNA polymerase gene, which is anticipated to be highly conserved. Since information on antigenic relationship is limited, due to the lack of a tissue culture system for these viruses, strains mostly are described on the basis of their genetic relatedness. However, the lack of uniformly… 

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Murine Noroviruses Comprising a Single Genogroup Exhibit Biological Diversity despite Limited Sequence Divergence

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A novel method, which allows simultaneous detection and genotyping of NLVs, is useful in the diagnosis and typing ofNLVs obtained from outbreaks and in large-scale epidemiological studies.

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Evidence is provided to support the theory of the role of subgenomic RNA promoters as recombination hotspots and a simple mechanism of how recombination might occur in NoV is described.

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Norwalk-like viruses (NLVs) were the most common cause of the gastroenteritis outbreaks investigated here, and the nucleotide sequence of part of the capsid gene (ORF2) from three of these strains demonstrated their relationship with Mexico virus.



Molecular characterization and expression of the capsid protein of a Norwalk-like virus recovered from a Desert Shield troop with gastroenteritis.

Immunoprecipitation studies and sequence analysis indicated the presence of shared antigenic sites between the capsid proteins of DSV395 and NV, however, hyperimmune sera specific for the self-assembled recombinant NV capsid protein did not react with DSV stool antigen in an ELISA, suggesting that there may also be unique antigenic Sites not shared between DSV 395 and NV.

Molecular characterization of Hawaii virus and other Norwalk-like viruses: evidence for genetic polymorphism among human caliciviruses.

Comparison of the amino acid sequence of the capsid protein of HV with NV and other human caliciviruses demonstrated the existence of two major genetic groups (genogroups) typified by HV and NV, and showed that there were these two genogroups.

Human calicivirus genogroup II capsid sequence diversity revealed by analyses of the prototype Snow Mountain agent

It is shown that the capsid proteins of OTH-25 and SMA are more distinct than was predicted by homology in the RNA polymerase, suggesting that recombination has occurred within this group of viruses.

Norwalk-like viruses: demonstration of genomic diversity by polymerase chain reaction

Evidence of a genomic relationship between Norwalk virus and serotype UK2 small round-structured viruses is provided, suggesting that viruses closely related to Norwalkirus have not been circulating widely in the United Kingdom in recent years.

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Sequence and genomic organization of Norwalk virus.

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Application of PCR to detect Norwalk virus in fecal specimens from outbreaks of gastroenteritis

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