Genetic polymorphism across regions of the three open reading frames of “Norwalk-like viruses”

@article{Vinj2000GeneticPA,
  title={Genetic polymorphism across regions of the three open reading frames of “Norwalk-like viruses”},
  author={Jan Vinj{\'e} and Jonathan Green and D. Lewis and Chris I. Gallimore and D. W. G. Brown and Marion P. G. Koopmans},
  journal={Archives of Virology},
  year={2000},
  volume={145},
  pages={223-241}
}
Summary. Genomic characterization of Norwalk-like human caliciviruses (NLVs) originating from outbreaks and sporadic cases of acute gastroenteritis has revealed surprisingly high levels of diversity, even in the RNA polymerase gene, which is anticipated to be highly conserved. Since information on antigenic relationship is limited, due to the lack of a tissue culture system for these viruses, strains mostly are described on the basis of their genetic relatedness. However, the lack of uniformly… Expand
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References

SHOWING 1-10 OF 37 REFERENCES
Geographic distribution of wild poliovirus type 1 genotypes.
TLDR
Comparing 150 bases of genomic sequence information from 62 isolates obtained from poliomyelitis patients in five continents allowed us to identify numerous geographic foci of endemic circulation of wild type 1 polioviruses, reveal previously unsuspected links between cases in distant communities, and recognize the recombinant (vaccine-wild; wild-wild) origins of some epidemic poliovIRuses. Expand
Molecular characterization and expression of the capsid protein of a Norwalk-like virus recovered from a Desert Shield troop with gastroenteritis.
TLDR
Immunoprecipitation studies and sequence analysis indicated the presence of shared antigenic sites between the capsid proteins of DSV395 and NV, however, hyperimmune sera specific for the self-assembled recombinant NV capsid protein did not react with DSV stool antigen in an ELISA, suggesting that there may also be unique antigenic Sites not shared between DSV 395 and NV. Expand
Molecular characterization of Hawaii virus and other Norwalk-like viruses: evidence for genetic polymorphism among human caliciviruses.
TLDR
Comparison of the amino acid sequence of the capsid protein of HV with NV and other human caliciviruses demonstrated the existence of two major genetic groups (genogroups) typified by HV and NV, and showed that there were these two genogroups. Expand
Human calicivirus genogroup II capsid sequence diversity revealed by analyses of the prototype Snow Mountain agent
TLDR
It is shown that the capsid proteins of OTH-25 and SMA are more distinct than was predicted by homology in the RNA polymerase, suggesting that recombination has occurred within this group of viruses. Expand
Norwalk-like viruses: demonstration of genomic diversity by polymerase chain reaction
TLDR
Evidence of a genomic relationship between Norwalk virus and serotype UK2 small round-structured viruses is provided, suggesting that viruses closely related to Norwalkirus have not been circulating widely in the United Kingdom in recent years. Expand
Human enteric Caliciviridae: a new prevalent small round-structured virus group defined by RNA-dependent RNA polymerase and capsid diversity.
TLDR
Computer analyses indicated that these viruses can be divided into two discrete groups, and sequence comparison of the RNA-dependent RNA polymerases of small round-structured viruses (SRSVs) revealed significant genetic variation. Expand
Sequence and genome organization of a human small round-structured (Norwalk-like) virus.
TLDR
The complete genomic nucleotide sequence of an SRSV, Southampton virus, was determined and it was found that the 7696-nucleotide RNA genome encodes three open reading frames whose sequences and organization strongly support proposals that SRVSs are members of the Caliciviridae. Expand
Expression and Self-Assembly of Grimsby Virus: Antigenic Distinction from Norwalk and Mexico Viruses
TLDR
The expression of the GRV capsid protein has allowed the antigenic comparison of three distinct recombinant Norwalk-like viruses for the first time, revealing that GRV is antigenically distinct from both NV and MXV. Expand
Sequence and genomic organization of Norwalk virus.
TLDR
The morphology, size, polarity, and genomic organization of the Norwalk virus indicate it is a member of the Caliciviridae family. Expand
Application of PCR to detect Norwalk virus in fecal specimens from outbreaks of gastroenteritis
TLDR
The successful application of RT-PCR to investigations of outbreaks of suspected NV-associated gastroenteritis will depend on the use of either multiple primer pairs or primers made against regions of the genome that are more conserved. Expand
...
1
2
3
4
...