Genetic organization of the mle locus and identification of a mleR-like gene from Leuconostoc oenos.

@article{Labarre1996GeneticOO,
  title={Genetic organization of the mle locus and identification of a mleR-like gene from Leuconostoc oenos.},
  author={C{\'e}cile Labarre and Charles Divi{\`e}s and Jean Guzzo},
  journal={Applied and environmental microbiology},
  year={1996},
  volume={62 12},
  pages={4493-8}
}
Characterization of the mle locus harboring the malolactic enzyme gene mleA and malate permease gene mleP from Leuconostoc oenos was completed in this study by mRNA analysis. Northern (RNA) blot experiments revealed a 2.6-kb transcript, suggesting an operon structure harboring mleA and mleP genes. Primer extension analysis showed that the mle operon has a single transcription start site located 17 nucleotides upstream of the ATG translation start site for the mleA gene. We found sequences… CONTINUE READING
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