Genetic organization of plasmid ColJs, encoding colicin Js activity, immunity, and release genes.

@article{majs2001GeneticOO,
  title={Genetic organization of plasmid ColJs, encoding colicin Js activity, immunity, and release genes.},
  author={David {\vS}majs and George Weinstock},
  journal={Journal of bacteriology},
  year={2001},
  volume={183 13},
  pages={3949-57}
}
The 5.2-kb ColJs plasmid of a colicinogenic strain of Shigella sonnei (colicin type 7) was isolated and sequenced. pColJs was partly homologous to pColE1 and to pesticin-encoding plasmid pPCP1, mainly in the rep, mob, and cer regions. A 1.2-kb unique region of pColJs showed significantly different G+C content (34%) compared to the rest of pColJs (53%). Within the unique region, seven open reading frames (ORFs) were identified. ORF94 was shown to code for colicin Js activity (cja), a 94-amino… CONTINUE READING

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