Genetic organization and transcription from the gene (trmA) responsible for synthesis of tRNA (uracil-5)-methyltransferase by Escherichia coli.

@article{Lindstrm1985GeneticOA,
  title={Genetic organization and transcription from the gene (trmA) responsible for synthesis of tRNA (uracil-5)-methyltransferase by Escherichia coli.},
  author={Peter Lindstr{\"o}m and Dietrich Stueber and Glenn R. Bj{\"o}rk},
  journal={Journal of bacteriology},
  year={1985},
  volume={164 3},
  pages={1117-23}
}
The enzyme catalyzing the formation of 5-methyluridine (ribothymidine) in tRNA of Escherichia coli is tRNA (uracil-5)-methyltransferase (EC 2.1.1.35). A 2.8-kilobase EcoRI chromosomal DNA fragment contains trmA, the structural gene for this enzyme. Subcloning, transcription in vitro, Tn5 insertion mutagenesis, and transcriptional fusion experiments were… CONTINUE READING