Genetic modification of human hematopoietic cells: preclinical optimization of oncoretroviral-mediated gene transfer for clinical trials.

@article{BudakAlpdogan2009GeneticMO,
  title={Genetic modification of human hematopoietic cells: preclinical optimization of oncoretroviral-mediated gene transfer for clinical trials.},
  author={Tulin Budak-Alpdogan and Isabelle Rivi{\`e}re},
  journal={Methods in molecular biology},
  year={2009},
  volume={506},
  pages={33-58}
}
This chapter provides information about the oncoretroviral transduction of human hematopoietic stem/ progenitor cells under clinically applicable conditions. We describe in detail a short -60 h transduction protocol which consistently yields transduction efficiencies in the range of 30-50% with five different oncoretroviral vectors. We discuss a number of… CONTINUE READING