Genetic method to identify regulons controlled by nonessential elements: isolation of a gene dependent on alternate transcription factor sigma B of Bacillus subtilis.

@article{Boylan1991GeneticMT,
  title={Genetic method to identify regulons controlled by nonessential elements: isolation of a gene dependent on alternate transcription factor sigma B of Bacillus subtilis.},
  author={Sharon A. Boylan and Michael D. Thomas and Chester W. Price},
  journal={Journal of bacteriology},
  year={1991},
  volume={173 24},
  pages={7856-66}
}
We describe a general, in vivo method for identifying Bacillus subtilis genes controlled by specific, nonessential regulatory factors. We establish the use of this approach by identifying, isolating, and characterizing a gene dependent on sigma B, an alternate transcription factor which is found early in stationary phase but which is not essential for sporulation. The method relies on two features: (i) a plate transformation technique to introduce a null mutation into the regulatory gene of… CONTINUE READING

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