Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome

@article{Varshney2006GeneticMA,
  title={Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome},
  author={Rajeev K. Varshney and Ivo Grosse and Urs Haehnel and Robert L Siefken and Manoj Prasad and Nils Stein and Peter Langridge and Lothar Altschmied and Andreas Graner},
  journal={Theoretical and Applied Genetics},
  year={2006},
  volume={113},
  pages={239-250}
}
A set of 111,090 barley expressed sequence tags (ESTs) was searched for the presence of microsatellite motifs [simple sequence repeat (SSRs)] and yielded 2,823 non-redundant SSR-containing ESTs (SSR–ESTs). From this, a set of 754 primer pairs was designed of which 525 primer pairs yielded an amplicon and as a result, 185 EST-derived microsatellite loci (EST–SSRs) were placed onto a genetic map of barley. The markers show a uniform distribution along all seven linkage groups ranging from 21 (7H… CONTINUE READING
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