Genetic manipulations restored the growth fitness of reduced-genome Escherichia coli.

@article{Hirokawa2013GeneticMR,
  title={Genetic manipulations restored the growth fitness of reduced-genome Escherichia coli.},
  author={Yasutaka Hirokawa and Hiroaki Kawano and Kimie Tanaka-Masuda and Noriko Nakamura and Akira Nakagawa and Mikito Ito and Hideo Mori and Taku Oshima and Naotake Ogasawara},
  journal={Journal of bioscience and bioengineering},
  year={2013},
  volume={116 1},
  pages={52-8}
}
Microbes with smaller genomes would be better chassis for analysis, design, and improvement in the fields of metabolic engineering, synthetic biology, and molecular breeding. To create an Escherichia coli strain with a smaller genome, we used a stepwise genome reduction approach. Beginning with strain MGF-01, which has a genome of 3.62 megabase pairs (Mbp), we generated two E. coli K-12 strains without any insertion sequence (IS), DGF-327 and DGF-298, with reduced genome sizes of 3.27 and 2.98… CONTINUE READING