Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis.

@article{Swindell1996GeneticMO,
  title={Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis.},
  author={Simon Swindell and Kathrine Benson and Hugh Griffin and Pierre Renault and Stanislas Dusko Ehrlich and Michael J. Gasson},
  journal={Applied and environmental microbiology},
  year={1996},
  volume={62 7},
  pages={2641-3}
}
Diacetyl is an important food flavor compound produced by certain strains of citrate-metabolizing lactic acid bacteria. Citrate is converted to pyruvate, from which diacetyl is produced via intermediate alpha-acetolactate. This paper reports the cloning and analysis of the gene (aldB) encoding alpha-acetolactate decarboxylase from Lactococcus lactis MG1363. Deletion of the MG1363 chromosomal aldB gene was achieved by double crossover homologous recombination. The mutant strain was found to… CONTINUE READING