Genetic manipulation of mouse embryonic stem cells by mutant lambda integrase.

@article{Christ2002GeneticMO,
  title={Genetic manipulation of mouse embryonic stem cells by mutant lambda integrase.},
  author={Nicole Christ and Peter Droege},
  journal={Genesis},
  year={2002},
  volume={32 3},
  pages={203-8}
}
Mutant lambda integrases catalyze site-specific recombination reactions inside mammalian cells. Here we demonstrate that the integrase system can be used to eliminate resistance marker genes from the genome of mouse embryonic stem cells. So-called integrative and excisive recombination pathways led to the precise deletion of the neomycin gene, which was inserted together with a flanking pair of directly repeated recombination sites into the ROSA26 locus by standard targeting techniques. The… CONTINUE READING