Genetic manipulation of Lactococcus lactis by using targeted group II introns: generation of stable insertions without selection.

@article{Frazier2003GeneticMO,
  title={Genetic manipulation of Lactococcus lactis by using targeted group II introns: generation of stable insertions without selection.},
  author={Courtney L. Frazier and Joseph San Filippo and Alan M. Lambowitz and David A. Mills},
  journal={Applied and environmental microbiology},
  year={2003},
  volume={69 2},
  pages={1121-8}
}
Despite their commercial importance, there are relatively few facile methods for genomic manipulation of the lactic acid bacteria. Here, the lactococcal group II intron, Ll.ltrB, was targeted to insert efficiently into genes encoding malate decarboxylase (mleS) and tetracycline resistance (tetM) within the Lactococcus lactis genome. Integrants were readily… CONTINUE READING