Genetic interaction mapping and exon-resolution functional genomics with a hybrid Cas9–Cas12a platform

@article{GonatopoulosPournatzis2020GeneticIM,
  title={Genetic interaction mapping and exon-resolution functional genomics with a hybrid Cas9–Cas12a platform},
  author={Thomas Gonatopoulos-Pournatzis and Michael Aregger and Kevin R. Brown and Shaghayegh Farhangmehr and Ulrich Braunschweig and Henry N. Ward and Kevin C. H. Ha and Alexander Weiss and Maximilian Billmann and Tanja Durbic and Chad L. Myers and Benjamin J. Blencowe and Jason Moffat},
  journal={Nature Biotechnology},
  year={2020},
  volume={38},
  pages={638-648}
}
Systematic mapping of genetic interactions (GIs) and interrogation of the functions of sizable genomic segments in mammalian cells represent important goals of biomedical research. To advance these goals, we present a CRISPR (clustered regularly interspaced short palindromic repeats)-based screening system for combinatorial genetic manipulation that employs coexpression of CRISPR-associated nucleases 9 and 12a (Cas9 and Cas12a) and machine-learning-optimized libraries of hybrid Cas9–Cas12a… 

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