Genetic instability of Japanese encephalitis virus cDNA clones propagated in Escherichia coli


The genetic instability of Flavivirus cDNA clones in transformed bacteria is a common phenomenon. Herein, a cDNA fragment of the nucleotide (nt) 1–2913 of the genome of a flavivirus, Japanese encephalitis virus (JEV), was used to investigate factors that caused the instability of cDNA clones. Several cDNA fragments with different 5′- or 3′-termini of the… (More)
DOI: 10.1007/s11262-016-1289-y


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