Genetic incorporation of d-lysine into diketoreductase in Escherichia coli cells

@article{Liu2012GeneticIO,
  title={Genetic incorporation of d-lysine into diketoreductase in Escherichia coli cells},
  author={Zhizhi Liu and Xin Yang and Denghuan Yi and Shuzhen Wang and Yijun Chen},
  journal={Amino Acids},
  year={2012},
  volume={43},
  pages={2553-2559}
}
Pyrococcus horikoshii lysyl-tRNA synthetase/tRNA orthogonal pair exhibited high selectivity towards d-lysine in the presence of excess amount of d-lysine. Based on the observation, this orthogonal pair was employed to encode d-lysine, and d-lysine was site-specifically incorporated into the diketoreductase in E. coli cells.