Genetic improvement of Escherichia coli for enhanced biological removal of phosphate from wastewater.

@article{Kato1993GeneticIO,
  title={Genetic improvement of Escherichia coli for enhanced biological removal of phosphate from wastewater.},
  author={Junichi Kato and Kenichiro Yamada and Atsushi Muramatsu and Hardoyo and Hisao Ohtake},
  journal={Applied and environmental microbiology},
  year={1993},
  volume={59 11},
  pages={3744-9}
}
The ability of Escherichia coli MV1184 to accumulate inorganic phosphate (Pi) was enhanced by manipulating the genes involved in the transport and metabolism of Pi. The high-level Pi accumulation was achieved by modifying the genetic regulation and increasing the dosage of the E. coli genes encoding polyphosphate kinase (ppk), acetate kinase (ackA), and the phosphate-inducible transport system (pstS, pstC, pstA, and pstB). Acetate kinase was employed as an ATP regeneration system for… CONTINUE READING

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