Genetic evidence that antibacterial activity of lysozyme is independent of its catalytic function

@article{Ibrahim2001GeneticET,
  title={Genetic evidence that antibacterial activity of lysozyme is independent of its catalytic function},
  author={H. Ibrahim and T. Matsuzaki and T. Aoki},
  journal={FEBS Letters},
  year={2001},
  volume={506}
}
  • H. Ibrahim, T. Matsuzaki, T. Aoki
  • Published 2001
  • Biology, Medicine
  • FEBS Letters
  • A catalytically inactive mutant of hen egg white lysozyme was constructed by site‐directed mutagenesis to elucidate the role of enzymatic activity on its antimicrobial activity against Gram‐positive bacteria. The catalytic residue aspartic acid at position 52 of lysozyme was substituted with serine (D52S‐Lz) and the mutant cDNA was inserted into a yeast expression vector, pYES‐2. Western blot analysis indicated that the mutation did not affect secretion of the D52S‐Lz lysozyme into the medium… CONTINUE READING
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