Genetic evidence for involvement of two distinct nonhomologous end-joining pathways in repair of topoisomerase II-mediated DNA damage.

@article{Adachi2004GeneticEF,
  title={Genetic evidence for involvement of two distinct nonhomologous end-joining pathways in repair of topoisomerase II-mediated DNA damage.},
  author={Noritaka Adachi and Susumu Iiizumi and Sairei So and Hideki Koyama},
  journal={Biochemical and biophysical research communications},
  year={2004},
  volume={318 4},
  pages={856-61}
}
In vertebrate cells, DNA double-strand breaks are efficiently repaired by homologous recombination or nonhomologous end-joining (NHEJ). The latter pathway relies on Ku (the Ku70/Ku86 heterodimer), DNA-PKcs, Artemis, Xrcc4, and DNA ligase IV (Lig4). Here, we show that a human pre-B cell line nullizygous for Lig4 exhibits hypersensitivity to topoisomerase II… CONTINUE READING