Genetic engineering of glomerular sclerosis in the mouse via control of onset and severity of podocyte-specific injury.

@article{Matsusaka2005GeneticEO,
  title={Genetic engineering of glomerular sclerosis in the mouse via control of onset and severity of podocyte-specific injury.},
  author={Taiji Matsusaka and Jing Xin and Suguri Niwa and Kazuto Kobayashi and Akira Akatsuka and Hiroomi Hashizume and Qing-cheng Wang and Ira Pastan and Agnes B. Fogo and Iekuni Ichikawa},
  journal={Journal of the American Society of Nephrology : JASN},
  year={2005},
  volume={16 4},
  pages={1013-23}
}
This study aimed to generate a mouse model of acquired glomerular sclerosis. A model system that allows induction of podocyte injury in a manner in which onset and severity can be controlled was designed. A transgenic mouse strain (NEP25) that expresses human CD25 selectively in podocytes was first generated. Injection of anti-Tac (Fv)-PE38 (LMB2), an immunotoxin with specific binding to human CD25, induced progressive nonselective proteinuria, ascites, and edema in NEP25 mice. Podocytes showed… CONTINUE READING