Genetic engineering of Escherichia coli to produce a 1:1 complex of the anabaena sp. PCC 7120 nuclease NucA and its inhibitor NuiA.

@article{Korn2000GeneticEO,
  title={Genetic engineering of Escherichia coli to produce a 1:1 complex of the anabaena sp. PCC 7120 nuclease NucA and its inhibitor NuiA.},
  author={Christian Understanding’Eugene Korn and Gregor Meiss and Frank-Ulrich Gast and Oleg A. Gimadutdinow and Claus Urbanke and Alfred Pingoud},
  journal={Gene},
  year={2000},
  volume={253 2},
  pages={221-9}
}
A series of T7-promoter based bicistronic expression vectors was constructed in order to produce the complex of the Anabaena sp. PCC 7120 DNA/RNA non-specific nuclease NucA and its inhibitor NuiA. With all constructs, tandem expression of nucA and nuiA results in aggregation and inclusion body formation of NucA, independent of the order of the genes, the relative expression of the two proteins and the temperature applied during expression. Two constructs in which nuiA is the first and nucA the… CONTINUE READING

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