Genetic engineering modification and fermentation optimization for extracellular production of recombinant proteins using Escherichia coli

@article{Zhou2018GeneticEM,
  title={Genetic engineering modification and fermentation optimization for extracellular production of recombinant proteins using Escherichia coli},
  author={Yuling Zhou and Zhenghui Lu and Xiang Wang and Jonathan Nimal Selvaraj and Guimin Zhang},
  journal={Applied Microbiology and Biotechnology},
  year={2018},
  volume={102},
  pages={1545-1556}
}
As a common expression host, Escherichia coli has received more and more attention due to the recently developed secretory expression system, which offers advantages like reduced downstream bioprocesses and improved product quality. These advantages, coupled with high-density fermentation technology, make it a preferred system for large-scale production of many proteins utilized in industry and agriculture at a reduced process cost. To improve the secretion efficiency of target proteins… 

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